{line}
{name}
{line}

Step 1 Prepare vector
.....................

Linearize `pYPKpw <./pYPKpw.txt>`_ with `EcoRV <http://rebase.neb.com/rebase/enz/EcoRV.html>`_
resulting in the `linearized vector <./pYPKpw_lin.txt>`_.

Step 2 tp-gene-tp PCR reactions
...............................

Perform the following {number} PCR reactions:

{pcrs}


Step 3 Transformation and Assembly
..................................

Mix the DNA fragments and transform a S. cerevisiae ura3 mutant. The DNA fragments 
will be assembled by in-vivo homologous recombination:
::

{figure}



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